They won in 37.47 seconds, with the U.S. taking silver at 0.05sec and Japan with a surprise bronze.
Bolt was attended to by medics but refused a ride in a wheelchair off the track, instead finally getting up and limping alongside his teammates through to the finish line and then off into the bowels of the stadium for treatment.
One night full of emotions and farewells has ended in London.
There was similar heartbreak for Shaunae Miller-Uibo, who had already missed out on a medal after she broke down towards the end of the individual 400m, when her Bahamas team failed to finish their relay heat. Mitchell-Blake was in tears and could barely speak. She lost for the first time since shockingly failing to make the Rio team at last year's Olympic Trials.
"He didn't tell us exactly what happened but from what I saw, it looked like a strain or a cramp of some sort", teammate Julian Forte told IAAF radio. So, when the Other Bolt got the baton in third place on the 4x100 anchor leg, it was clear he was not going to win. "We smashed the British record to pieces." said Mitchell-Blake.
100m champion Justin Gatlin called Bolt "still the best in the world" after he won silver behind Great Britain, and also felt that the weather may have played a part in Bolt's injury.
While Britain celebrated, though, there were contrasting emotions for Bolt in the final race of his career.
The 30-year-old, after an initial sprint, started hobbling and ended up rolling on the track with a hamstring injury, according to reports. He hauls himself up and limps across the line before applauding the crowd and crouching back down in some pain.
"It's sad to see", Talbot said of Bolt's ending, which was put down to hamstring cramp. He'll be ready. He has a passion for the sport, he loves the fans, the fans love him. "He's the greatest of all time".
Team Jamaica was facing off against a tough field with Team USA and Great Britain.
"The sport is going to drop a bit (following the departure of Bolt) but we still have guys for Jamaica to fill that void that he will leave". They beat Jamaican by 0.07s. This was a step up again from a team with an average age of 22. Olympic champion Thomas Roehler of Germany finished fourth.
"I think it was the elements (that caused the injury)", he said.
Having gone through their meticulous warm ups, the teams were made to wait as the medal presentations for the men's 5000 metres and women's high jump were carried out.